95,838 results

  • Long Bay marine monitoring program: 1999-2010. Prepared by University of Auckland for Auckland Regional Council

    Shears, Nicholas (2010)

    Report
    The University of Auckland Library

    The Long Bay Marine Monitoring Programme (LBMMP) was established in 1999 to detect and document the impact of urban development at Long Bay on the subtidal marine environment. The LBMMP involves annual sampling of shallow subtidal reef communities at 30 rocky reef sites located between Campbells Bay and Waiwera, and monthly measurements of sedimentation at each site using sediment traps.

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  • Meola Reef ecological monitoring program: 2001-2010. Prepared by University of Auckland for Auckland Regional Council.

    Shears, Nicholas (2010)

    Report
    The University of Auckland Library

    Monitoring of biological communities at six intertidal and six subtidal rocky reef sites has been carried out at Meola Reef in Auckland???s Waitemata Harbour since 2001. In addition, sedimentation rates have been monitored on a monthly basis at the six subtidal sites over this period. The primary goal of this State of the Environment monitoring program is to document long-term trends in community composition and interpret these changes in relation to major threats to the harbours marine environment; primarily sedimentation and toxic urban discharge.

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  • Protein and gene expression analyses in bone marrow stem cells mediated restoration of myocardium after ischemic insult

    Lee, Kathryn (2010)

    Doctoral thesis
    The University of Auckland Library

    Myocardial Infarction (MI) is caused by occlusion of the coronary artery following atheromatous plaque rupture, the subsequent ischemia in the myocardium leads to myocyte necrosis unless treated quickly. Bone marrow derived stem cell treatment is a promising therapy for improving the outcome of patients with MI. The aim of this thesis was to study myocardial protein and gene expression changes in a rat ischemia/reperfusion (I/R) model in order to look for potential repair mechanisms of the myocardium triggered by endogenous bone marrow mononuclear cells (BMMNCs). Rat myocardial samples were obtained from three experimental groups: one group had a sham operation, the other two groups had undergone myocardial I/R injury induced by left anterior descending (LAD) coronary artery ligation followed by treatment with either a BMMNC preparation or PBS. Comparative proteomic analyses were carried out using 2D electrophoresis; differentially expressed proteins were identified using LC-MS/MS. Western blotting was used to confirm the most significant findings including expression of 14-3-3 epsilon protein. Global comparative gene expression profiling was performed using Illumina RatRef12 BeadChips and QPCR was used to validate the top results. Bioinformatic tools were used to assess the biology of the differentially expressed genes and proteins. Thirty-seven proteins were found to be differentially expressed in I/R injury compared to sham. These were primarily sarcomeric, energy production or stress response proteins and most were down-regulated. Expression levels were ???corrected??? by BMMNC treatment for many of these proteins. Over 1500 genes were affected by I/R injury, 20 were affected by BMMNC treatment, and many of these were related to inflammation and apoptosis signalling and responses. The 14-3-3 epsilon protein was chosen for follow-up work as it presented as a good candidate for mechanistic involvement. This protein has many roles including interactions with the proapoptotic BCL2-associated agonist of cell death (Bad) protein. Western blotting was used to look at Bad expression and found it to be significantly increased in the treatment group, although I could not reliably measure the expression of phosphorylated (serine 136) form of Bad. A preliminary pull-down assay was performed to look for binding partners of 14-3-3 epsilon. Two ATP synthase subunits, one of which is known to bind 14-3-3 epsilon, a protein involved in fatty acid ??-oxidation and a protein of unknown function were found to bind. Further work will be required to follow up these findings and ascertain the exact role of 14-3- 3 epsilon in cardioprotection. In summary, my data supports the power of profiling methods to derive new candidates for a role in repair mechanisms in this therapeutic model.

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  • Recent Advances in Cytometry, Part B: Advances in Applications

    Wlodkowic, Donald (2011)

    Book
    The University of Auckland Library

    This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics.

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  • Recent Advances in Cytometry, Part A: Instrumentation, Methods

    Wlodkowic, Donald (2011)

    Book
    The University of Auckland Library

    An updated edition of the classic Methods in Cell Biology volume 48, this book, along with its companion volume, emphasizes diverse methods and technologies ...

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  • Selective targeting of apoptotic pathways in follicular lymphoma cells

    Wlodkowic, Donald (2007)

    Book
    The University of Auckland Library

    The seminal discovery that the BCL2 gene inhibits cell death rather than promotes cell proliferation gave the foundation for a nowadays widely appreciated theory that impaired tumor cell death is a decisive stage in a multi-step carcinogenesis. Recognition of the central role of mitochondria and Bcl-2 family members in the regulation and propagation of cell demise has, thus, recently uncovered novel targets for selective anti-cancer therapies. In this context, we have for the first time demonstrated monotherapeutic potential of a small molecule Bcl-2 antagonist, HA14-1, against follicular lymphoma (FL) cells with t(14;18) translocation and Bcl-2 overexpression. In agreement with others we have shown that cytotoxicity mediated by a small molecule Bcl-2 inhibitor HA14-1 in FL cells proceeds through rapid (4h) dissipation of the ∆Ψm, generation of ROS and caspase-dependent apoptosis. Moreover, we were first to prove the applicability of combinatorial treatment of HA14-1 and selected conventional chemotherapeutics (dexamethasone and doxorubicin) in FL cells. In the following work we investigated the quantitative relationship between ∆Ψm loss and caspase activation in HA14-1 treated cells. Employing both pharmacological inhibitor studies and novel state-of-the-art multiparametric flow cytometry assays, we revealed that following HA14-1 treatment caspase activation occurs solely as a consequence of mitochondrial breach. Additionally, we provided new evidence that HA14-1-evoked apoptosis appears to be at least partially PT-dependent. We also for the first time addressed the cell cycle specificity of HA14-1 action using multivariate flow cytometry approaches. As the interest in the role of ER and Golgi during induction/execution of apoptosis has been gaining momentum, they simultaneously attract growing appreciation in the development of novel anti-cancer therapies. Pertinent to the therapy of B-cell malignancies we report here for the first time the effects of an ER-Golgi transport inhibitor, Brefeldin A (BFA), alone and in combination with a small molecule Bcl-2 inhibitor HA14-1 or death receptor trigger, in the human FL cell lines bearing t(14;18) translocation. Of importance for future anti-cancer regimens, small molecule Bcl-2 antagonist, HA14-1 and agonistic anti-Fas mAb significantly enhanced BFA- mediated cytotoxicity and apoptosis, revealing novel and previously unexplored avenues to enhance ER-stress mediated cell killing in B-cell malignancies. Finally, as basic studies advance towards their ultimate translational goals there is a need for effective and rapid analytical methods allowing high-throughput detection of diverse cell demise modes. In this context patented SYTO® probes are gaining increasing interest as easy to use markers of apoptosis. Herein, applying the state-of-the-art multiparametric flow cytometry and multicolor cell imaging we identified for the first time different SYTO16 staining characteristics upon apoptotic and oncotic stimuli. We also tracked similarities and discrepancies between SYTO16 and ∆Ψm, sensitive probe, tertramethylrhodamine methyl ester (TMRM), demonstrating that stimulation with mitochondrial uncoupler FCCP and a small-molecule Bcl-2 inhibitor, HA14-1, induce distinct staining profiles with the decrease in TMRM fluorescence preceding the loss of SYTO16 fluorescence. To our knowledge this is the first report demonstrating such a distinct behavior of SYTO16 and TMRM and our data demonstrate that loss of SYTO16 is caspase-dependent, as is not a mere indicator of ∆ψm dissipation, postulated previously by some authors.

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  • Growing research in practice: A collection of resources

    Fouche, Christa; Lunt, N; Yates, D (2007)

    Book
    The University of Auckland Library

    introduction 0.1 What is Growing Research In Practice? Growing Research In Practice (GRIP) has consisted of an experimental programme of workshops and mentoring to eight (initially nine) groups of practitioner researchers in social services across the Auckland region of New Zealand. Funded by the Families Commission and SPEaR (Social Policy Evaluation and Research Fund of the Ministry of Social Development) and by the ASB Trust in partnership with the ANZASW (Aotearoa New Zealand Association of Social Work) it was the brainchild initially of Neil Lunt (then Massey University, now University of York) and Christa Fouch?? (Massey University) in 2005, who were shortly joined by Liz Beddoe and Phil Harington of Auckland University. ...

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  • Inhibitors for Desktop Parallelisation

    Giacaman, Nasser; Sinnen, Oliver (2006)

    Report
    The University of Auckland Library

    Parallel computing is notoriously challenging, making it dif???cult to develop ef???cient and correct programs. With the arrival of multicore processors, desktop environments must be parallelised if they are to bene???t from these new processors. However, the parallelisation of desktop environments entails even more challenges than that in a typical parallel program. This report outlines such challenges, suggesting possible areas and solutions to investigate.

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  • Parallel Task for parallelising object-oriented desktop applications

    Giacaman, N; Sinnen, O (2010)

    Report
    The University of Auckland Library

    As multi-cores arrive for mainstream desktop systems, developers must invest the effort to parallelize their applications. We present Parallel Task (short ParaTask), a solution to assist the parallelization of object-oriented applications, with the unique feature of including support for the parallelization of graphical user interface (GUI) applications. In the simple, but common, cases concurrency is introduced with a single keyword. Due to the wide variety of parallelization needs, ParaTask integrates different task types into the same model, provides intuitive support for dependence handling, non-blocking notification, interim progress notification and exception handling in an asynchronous environment as well as supporting a pluggable task scheduling runtime (currently work-sharing, work-stealing and a combination of the two are supported). The performance is compared to traditional Java parallelization approaches using a variety of different workloads.

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  • E noho marae - transforming learning through direct M??ori cultural experience

    Legge, Maureen (2010)

    Book item
    The University of Auckland Library

    This chapter is about the transformational learning of five day marae stays with physical education teacher education students. An initial marae stay with physical education student teachers in 1993 became a significant event that helped to shape and inform a curriculum innovation to include a broader experience of M??ori culture in a degree in physical education. In this chapter using writing as a method of inquiry (Richardson, 2000) I situate the context of my work in physical education and outdoor education teacher education; illustrate my ???slant??? to teaching through experiential learning (Kolb, 1984) in a marae-based context in partnership with M??ori outdoor educators as a link to teaching physical education???s te reo kori; show facets of student experience on the marae; and reveal how various outlooks of the tangata whenua and P??keh?? expertise come together to inform and shape the physical education students experience of M??oritanga. Kolb, D. (1984). Experiential learning. Englewood Cliffs, NJ: Prentice Hall. Richardson, L. (2000). Writing: A method of inquiry. In N. Denzin & Y. Lincoln (Eds.), Handbook of Qualitative Research (pp. 923-948). Thousand Oaks, CA: Sage Publications.

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  • The application of data mining tools and statistical techniques to identify patterns and changes in fire events

    Holmes, Mark; Wang, Yong; Ziedins, Ilze (2009)

    Report
    The University of Auckland Library

    This study explores the extent to which data mining and statistical techniques might assist the Fire Service in detecting threshold and pattern changes in its spatio-temporal fire data. Three entirely different scenarios are investigated. A post-hoc search for patterns was made of fires of suspicious or unknown cause in an area where a subsequently convicted arsonist was known to be operating. The spatio-temporal occurrence of chimney fires was compared with local climate data looking for any threshold conditions which might trigger the seasonal changes in occurrence. Finally an attempt is made to measure the effectiveness of the Firewise programme, which involves fire fighters visiting schools to instruct students in fire safety. The before and after incidence of residential fires in proximity to schools visited is assessed to determine whether the programme has had any measurable effect. Different data mining techniques are applied to each scenario.

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  • Robotic long bone fracture reduction

    Xie, SQ; Graham, AE; AW, KC; Xu, Peter; Mukherjee, S (2008)

    Book item
    The University of Auckland Library

    1. Introduction Medical robotics is still a relatively new field with researchers and companies all adopting various styles and techniques to solve the challenges faced. This chapter outlines one unique approach to the development of a medical robot for the reduction of broken femurs....

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  • A Parallel Navier-Stokes Solver for Natural Convection and Free Surface Flow

    Norris, Stuart (2001)

    Doctoral thesis
    The University of Auckland Library

    A parallel numerical method has been implemented for solving the Navier Stokes equations on Cartesian and non-orthogonal meshes. To ensure the accuracy of the code first, second and third order differencing schemes, with and without flux-limiters, have been implemented and tested. The most computationally expensive task in the code is the solution of linear equations, and a number of linear solvers have been tested to determine the most efficient. Krylov space, incomplete factorisation, and other iterative and direct solvers from the literature have been implemented, and have been compared with a novel black-box multigrid linear solver that has been developed both as a solver and as a preconditioner for the Krylov space methods. To further reduce execution time the code was parallelised, after a series of experiments comparing the suitability of different parallelisation techniques and computer architectures for the Navier Stokes solver. The code has been applied to the solution of two classes of problem. Two natural convection flows were studied, with an initial study of two dimensional Rayleigh Benard convection being followed by a study of a transient three dimensional flow, in both cases the results being compared with experiment. The second class of problems modelled were free surface flows. A two dimensional free surface driven cavity, and a two dimensional flume flow were modelled, the latter being compared with analytic theory. Finally a three dimensional ship flow was modelled, with the flow about a Wigley hull being simulated for a range of Reynolds and Froude numbers.

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  • Mastication Robots - Biological Inspiration to Implementation

    Xu, Peter; Bronlund, John E (2010-03)

    Book
    The University of Auckland Library

    This book is a valuable reference for researchers, engineers and graduates in the field of robotics, mechatronics, automatic control, artificial intelligence ...

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  • Preterm Labour and Delivery.

    Groom, Katie (2010)

    Book item
    The University of Auckland Library

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  • Bicultural perspectives of education outdoors: Part 1: A P??keh?? perspective on biculturalism in education outdoors

    Legge, Maureen (2012)

    Book item
    The University of Auckland Library

    The New Zealand historian Michael King (1999) wrote that M??ori had every right to be able to be M??ori in their own country and to expect P??keh?? to respect them. This principle underpins my work as a teacher educator in physical education and outdoor education. Like many P??keh?? New Zealanders, on a day-to-day basis I am remote from M??ori ritual and cultural practices. This is a severe limitation, so on a personal and professional level I have had to make a conscious effort to understand M??ori culture and to develop any sense of (bi)-cultural competency. In this chapter I narrate, and critique, the nature and shape of cultural borders I have crossed in order to negotiate bicultural teaching and learning, and build M??ori-P??keh?? educative partnerships in the outdoors. My outdoor educative partnerships centre on marae stays in Tai Tokerau Northland where the tangata whenua teach local history and issues that are important to them, specifically locating the impact of colonisation and the Treaty of Waitangi. The turangawaewae of the marae and local places of sea and forest, are the means to learn and practice tikanga M??ori. Away from the marae, I endeavor to include elements of M??ori tikanga in my pedagogy. However, the challenges implicit in ???being P??keh????? (King 1999, p. 9) and committed to M??ori cultural practices in education outdoors run the risk of being immobilised in the face of postcolonial politics. I identify some of the tensions and contestation embedded in being P??keh??, and being bicultural in education outdoors. Looking to the future, I critically reflect on some caveats for developing a biculturally responsive pedagogy in education outdoors. King, M. (1999). Being Pakeha now: Reflections and recollections of a white native. Auckland: Penguin Books.

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  • The shifting sands of tertiary individual consultation

    Carter, Susan (2010-11-18)

    Conference item
    The University of Auckland Library

    Tertiary individual consultation entails teaching and learning at its most personally situated: two people engage in tailor-made learning. The closed-door practices of supervision have had an airing recently, yet the closed-door practice of general individual consultation remains for the most part just that, highly individual, with decisions about pedagogical practice occurring on the spot. A 2007 survey of Association of Tertiary Learning Advisors (TLAs) of Aotearoa/New Zealand (ATLAANZ) provided some evidence of current individual appointment work practice and opinions. A follow-up survey in 2009 revisited opinion on some of the issues that emerged both from the earlier survey and my own reflection on the issues that it surfaced. Under the shifting sands of changing practice, guidelines might firm pedagogy. This paper teases out the ethical issues of individual consultation from a TLA perspective. It proposes that narrative therapy questioning practice can be useful in tertiary consultation. The TLA survey and discussion about individual consultation is framed as addressing an area that can be problematic for all academics: individual appointments and the ethics that underpin practice.

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  • Use of self in practice: a framework for integrating personal and professional knowledge

    Marlowe, Jay; Chinnery, Shirley Ann (2011)

    Book item
    The University of Auckland Library

    Providing assignments that promote critical learning opportunities and encourage social work students to reflectively engage with 'self' whilst on placement represents a core component of field work education. This chapter discusses how the Eastern-informed practice of mindfulness can help students attune to connections between the diverse elements of knowledge through a 'use of self' assignment. ...

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  • Reflection on best practice: A kiwi perspective on 'new dimensions for doctoral programmes in Europe'

    Carter, Susan (2007)

    Conference item
    The University of Auckland Library

    The 2006 United Kingdom Council for Graduate Education (UKCGE) summer conference has implications for learning advisors who support doctoral students. European mapping of new dimensions of doctoral research affects Australian and New Zealand universities. This article reflects from a learning advisor???s perspective on the implications of some of the issues raised at the conference, such as increased doctoral output; equity; excellence; transferable skills; flexibility and the market model of the knowledge economy research boom. ???Best practice??? is a focus of the article. Nonetheless this term is found to be problematically stretched across a set of tensions inherent in the current desires and responsibilities of universities. Doctoral programme support is placed on ground zero of many of these tensions, with potential for growth along with some new challenges.

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  • Who are we? Aotearoa New Zealand learning advisors talk about themselves

    Carter, Susan; Bartlett-Trafford, J (2008)

    Conference item
    The University of Auckland Library

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