2 results for Petroff, MG

  • Minor Histocompatibility Antigens Are Expressed in Syncytiotrophoblast and Trophoblast Debris: Implications for Maternal Alloreactivity to the Fetus

    Holland, Olivia; Linscheid, C; Hodes, HC; Nauser, TL; Gilliam, M; Stone, Peter; Chamley, Lawrence; Petroff, MG (2012)

    Journal article
    The University of Auckland Library

    The fetal semi-allograft can induce expansion and tolerance of antigen-specific maternal T and B cells through paternally inherited major histocompatibility complex and minor histocompatibility antigens (mHAgs). The effects of these antigens have important consequences on the maternal immune system both during and long after pregnancy. Herein, we investigate the possibility that the placental syncytiotrophoblast and deported trophoblastic debris serve as sources of fetal mHAgs. We mapped the expression of four mHAgs (human mHAg 1, pumilio domain-containing protein KIAA0020, B-cell lymphoma 2-related protein A1, and ribosomal protein S4, Y linked) in the placenta. Each of these proteins was expressed in several placental cell types, including the syncytiotrophoblast. These antigens and two additional Y chromosome-encoded antigens [DEAD box polypeptide 3, Y linked (DDX3Y), and lysine demethylase5D] were also identified by RT-PCR in the placenta, purified trophoblast cells, and cord blood cells. Finally, we used a proteomic approach to investigate the presence of mHAgs in the syncytiotrophoblast and trophoblast debris shed from first-trimester placenta. By this method, four antigens (DDX3Y; ribosomal protein S4, Y linked; solute carrier 1A5; and signal sequence receptor 1) were found in the syncytiotrophoblast, and one antigen (DDX3Y) was found in shed trophoblast debris. The finding of mHAgs in the placenta and in trophoblast debris provides the first direct evidence that fetal antigens are present in debris shed from the human placenta. The data, thus, suggest a mechanism by which the maternal immune system is exposed to fetal alloantigens, possibly explaining the relationship between parity and graft-versus-host disease.

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  • IFPA Meeting 2011 workshop report III: Placental immunology; Epigenetic and microRNA-dependent gene regulation; Comparative placentation; Trophoblast differentiation; Stem cells

    Ackerman IV, WE; Bulmer, JN; Lash, GE; Carter, AM; Chaillet, JR; Sadovsky, Y; Chamley, Lawrence; Holland, Olivia; Chen, CP; Chuong, EB; Coleman, SJ; Heazell, AE; Jones, CJP; Collet, GP; Southcombe, JH; Croy, BA; De Mestre, AM; Dickinson, H; Ducray, J; Enders, AC; Fogarty, NME; Moffett, A; Gauster, M; Huppertz, B; K??nig, J; Golos, T; Haider, S; Husebekk, A; John, RM; Johnsen, GM; Kalionis, B; Lorenzon, AR; Moreira De Mello, JC; Nuzzo, AM; Rolfo, A; Parham, P; Parolini, O; Petroff, MG; Pidoux, G; Ram??rez-Pinilla, MP; Robinson, WP; Soma, H; Tilburgs, T (2012-02)

    Journal article
    The University of Auckland Library

    Workshops are an important part of the IFPA annual meeting as they allow for discussion of specialised topics. At IFPA meeting 2011 there were twelve themed workshops, five of which are summarized in this report. These workshops related to various aspects of placental biology: 1) immunology; 2) epigenetics; 3) comparative placentation; 4) trophoblast differentiation; 5) stem cells. ?? 2012 Published by IFPA and Elsevier Ltd.

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