276 results for Conference poster

  • Substrate capture mechanism provides a mode for inhibition

    Evans, Genevieve; Short, F; Castell, A; Cookson, T; Gamage, Swarnalatha; Denny, B; Baker, E; Lott, S (2011-05-11)

    Conference poster
    The University of Auckland Library

    Mycobacteria tuberculosis (Mtb), the causative agent of tuberculosis, is responsible for more death in the world today than any other bacteria. As part of the Tuberculosis Structural Genomics Consortium (TBSGC), our research group previously determined the structure of anthranilate phosphoribosyl transferase (AnPRT) from Mtb. AnPRT is the second enzyme in the tryptophan biosynthetic pathway and was identified as a potential drug target through gene knockout experiments, which resulted in a strain of Mtb that was essentially avirulent even in immunodeficient mice. AnPRT catalyses a reaction between anthranilate and phosphoribosylpyrophosphate (PRPP), and the crystal structure of Mtb-AnPRT was originally determined with and without PRPP (PDB ID: 1ZVW and 2BPQ, respectively). In silico docking was used to predict the binding motif of anthranilate, the second substrate, surprisingly predicted two sites despite a 1:1 reaction ratio with PRPP. Previously, 165 compounds were screened for inhibitory action against Mtb-AnPRT. The most potent of these compounds was co-crystallized with Mtb-AnPRT and PRPP. One compound had a bianthranilate character and the 2.0 ?? resolution structure of this inhibitor bound to Mtb-AnPRT (PDB ID: 3QQS) was determined by molecular replacement using the Mtb-AnPRT structure without PRPP bound (PDB ID: 1ZVW) as a search model. Interestingly, the structure revealed multiple binding motifs for the inhibitor, two of which were consistent with the previously predicted binding motifs for anthranilate. Forty analogues of this potent Mtb-AnPRT inhibitor were subsequently assayed for activity against the enzyme, several of which showed were found to be more potent inhibitors. This new series of inhibitors were docked into the 3QQS structure, providing insights for the development of more potent inhibitors. Such techniques will continue to drive design of increasingly potent inhibitors against Mtb-AnPRT for future development of a new anti-tuberculosis agent.

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  • An investigation into the teaching of extemporaneous compounding skills to pharmacy students in schools of pharmacy in Australia, Canada, New Zealand, South Africa and the United Kingdom

    Aspden, Trudi; Rew, A; Anderson, C; Tan, J; Woodrow, R; Zheng, Y (2011)

    Conference poster
    The University of Auckland Library

    Background: Many countries require that registered pharmacists are competent to extemporaneously compound. In general, however, there is a lack of opportunity for interns and new pharmacists to practice extemporaneous compounding due to the reduction in demand for extemporaneously compounded products in community pharmacy. Thus it falls to schools of pharmacy to prepare future pharmacist for this role. Objectives: With respect to extemporaneous compounding to pharmacy undergraduates- to determine what is taught, how it is taught, how it is assessed and the time allocated to teaching in the different schools of pharmacy in the five countries. Methodology: Thirty eight course coordinators involved in the teaching of extemporaneous compounding from 32 schools of pharmacy in Australia, Canada, New Zealand, South Africa and the United Kingdom were invited to complete an online survey using Survey MonkeyTM. Results: Twenty four responses were obtained from participants in all five countries (response rate 63.2%). Extemporaneous compounding was a compulsory component of all the BPharm programmes. However, there was a wide inter and intra-country variation in the teaching of the subject including the number of hours dedicated to its teaching, the dosage forms included and the teaching methods used. Many schools used extemporaneous compounding teaching to introduce transferrable skills, such as the ability to accurately calculate. A strong desire to retain the teaching of extemporaneous compounding was expressed by the course coordinators. Discussion: Our results highlight differences in the extemporaneous compounding teaching of the BPharm programmes in the five countries surveyed, but also uncover its use in developing related skills.

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  • Blood flow redistribution following pulmonary micro-embolism

    Clark, Alys; Burrowes, KS; Tawhai, Merryn (2010)

    Conference poster
    The University of Auckland Library

    Occlusion of pulmonary arteries by autologous clot and bead emboli affect pulmonary function by elevating arterial pressures and reducing the number of functional gas exchange units in the lung. The occlusion of multiple arterioles at the acinar level can have a significant impact on pulmonary function. However, the contribution of acinar structure to perfusion distribution and the significance of arteriole occlusion is not well characterized.

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  • Pregnancy Associated Changes in Insulin Signalling in Daughters of Adolescent Ewes

    Oliver, Mark; Hancock, SN; Kenyon, PR; Blair, HT; Pain, S; Morris, S; Phua, Hui; Bloomfield, Francis (2011-09)

    Conference poster
    The University of Auckland Library

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  • A synthetic study towards aryl 6,6-spiroacetal analogues of rubromycin

    Choi, Peter; Rathwell, DC; Brimble, Margaret (2008)

    Conference poster
    The University of Auckland Library

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  • A Computational Model For Cerebral Circulation And Its Application For Haemodynamic Modelling In Vascular Surgeries

    Ho, Harvey; Mithraratne, K; Hunter, Peter (2009)

    Conference poster
    The University of Auckland Library

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  • A case study on frontline perspectives of organizational change: A practitioner-academic partnership to analyse the creation of a Youth Justice entity in Child, Youth and Family

    Webster, Michael; Herrmann, Klaus (2009-11-11)

    Conference poster
    The University of Auckland Library

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  • A frontline perspective of organizational change: Analyzing the creation of a Youth Justice entity in Child, Youth and Family using Lewin, Kotter & Schein's change management and organizational culture models

    Webster, Michael; Herrmann, K (2009-03-19)

    Conference poster
    The University of Auckland Library

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  • 'Yes we can!': The emerging contribution of social work leadership to clinical governance and quality improvement in district health board mental health services

    McNabb, D; Webster, Michael (2009)

    Conference poster
    The University of Auckland Library

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  • Orbscan And Pentacam Analysis Of The Cornea In Marfan And Suspected Marfan Syndrome.

    Vincent, Andrea; Ikink, W; Al-Taie, R (2011-05-02)

    Conference poster
    The University of Auckland Library

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  • The management of change in social services in Aotearoa New Zealand: An examination of the influence of post 1987 'new public managerialism, on organizational culture

    Webster, Michael (2009-03-19)

    Conference poster
    The University of Auckland Library

    New public managerialism??? has transformed organizational cultures of public/NGO sectors ???Government by contract??? inaugurated: Accountability for specified outputs Performance agreements between Ministers and Chief Executives Devolution of accountability required managers to develop leadership skills, strategic thinking, business planning, and performance management systems International Federation of Social Work recognition of management (???core purpose of social work???) professionally validates the project

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  • Clinical ICT Tools: Are we able to measure their effectiveness? A Case Study

    Ewens, Andrew; Orr, M; Starr Jr, RG (2014-09-10)

    Conference poster
    The University of Auckland Library

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  • Over-expression of Human Amylin Leads to Oligomerization and beta-cell Dysfunction Associated with Mitochondrial Uncoupling, Activation of c-Jun and Decrease Expression of JNK Interacting Protein-1

    Zhang, Shaoping; Liu, H; Chuang, CL; Li, XL; Au, M; Zhang, L; Cooper, GJS (2012-06-10)

    Conference poster
    The University of Auckland Library

    Over-expression of human amylin (hA) in pancreatic ??-cells has been shown to contribute to cytotoxic hA aggregation and islet amyloid formation that can lead to ??-cell dysfunction in type-2 diabetes mellitus. We aimed to investigate the functional and molecular changes associated with hA oligomer formation and their relation to ??-cell dysfunction and diabetes development using transgenic mouse model that over-expresses hA in their islet ??-cells.We showed that both homozygous and hemizygous hA transgenic mice developed spontaneous diabetes with different elevated levels of hA and with different time frame of disease onset and death. Homozygous mice displayed hyperinsulinemia and self-limiting insulin resistance during the pre-diabetic state, whereas by contrast, hemizygous mice had a longer prediabetic phase without insulin resistance. Intracellular and extracellular oligomers were clearly detectable before onset of diabetes with strong correlation with the time of ??-cell apoptosis occurred in homozygous but not in hemizygous mice, indicating a difference in the extent of cytotoxic oligomerization between these animals. In addition, RT-qPCR analysis demonstrated progressive decrease in ??-cell expression of functional and key regulatory molecules such as insulin, amylin, Pdx1, MafA, Glut2 and GCK. We also detected changes in expression of the mitochondrial membrane protein UCP-2 which contributes to decreased mitochondrial function. Further molecular analysis demonstrated activation of c-Jun/JNK and decrease expression of JNK-interacting protein 1, suggesting their role in mediating beta-cell death/apoptosis. Our studies should lead to a better understanding of the role and regulation of hA-evoked ??-cell dysfunction and ??-cell death in diabetes

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  • The role of conspicuity in bicycle crashes involving a motor vehicle

    Tin Tin, Sandar; Woodward, Alistair; Ameratunga, Shanthi (2014-10-30)

    Conference poster
    The University of Auckland Library

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  • Educating for disaster: Determining the core elements of a disaster curriculum for social work in New Zealand

    Adamson, Carole (2012)

    Conference poster
    The University of Auckland Library

    In 2010 and 2011, Aotearoa New Zealand was hit by a number of major disasters involving loss of human life and severe disruption to social, ecological and economic wellbeing. The Pike River mine explosions were closely followed by a sequence of major earthquakes in Christchurch, seismic events that have permanently altered the lives of thousands of people in our third largest city, the closure of the central business district and the effective abandonment of whole residential areas. In early October 2011, the ship, Rena, grounded on a reef off the port of Tauranga and threatened a major oil spill throughout the Bay of Plenty, where local communities with spiritual and cultural connections to the land depend on sea food as well as thrive on tourism. The Council for Social Work Education Aotearoa New Zealand (CSWEANZ), representing all the Schools of Social Work in New Zealand, held a ???Disaster Curriculum??? day in November 2011, at which social workers and Civil Defence leaders involved in the Christchurch earthquakes, the Rena Disaster, Fiji floods and the Boxing Day tsunami presented their narrative experience of disaster response and recovery. Workshops discussed and identified core elements that participants considered vital to a social work curriculum that would enable social work graduates in a range of community and cultural settings to respond in safe, creative and informed ways. We present our core ideas for a social work disaster curriculum and consider a wide range of educational content based on existing knowledge bases and new content within a disaster framework.

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  • "But what about the theory?" Designing a social work curriculum around practice learning and reflection

    Adamson, Carole; Bellinger, A (2010-06-10)

    Conference poster
    The University of Auckland Library

    Curriculum design in an academic context operates within a site of tension characterised by the need

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  • Resources and strategies used in New Zealand community pharmacies to identify and assist patients with low literacy: An opportunity to improve health outcomes.

    Aspden, Trudi; Sheridan, JL; McKie, J (2011)

    Conference poster
    The University of Auckland Library

    Background: Patients with lower literacy generally have less knowledge of health services, poorer health outcomes[1] and are more likely to have difficulty understanding prescription medication warning labels[2]. Objectives: To determine how pharmacy staff identify patients with limited literacy skills, the strategies used for identification, the resources available to help patients with low literacy and opportunities for upskilling. Methodology: A questionnaire was adapted from one developed by Praska et al 2005 [3]. A random sample of 120 New Zealand pharmacies were sent information about the study. Those pharmacists willing to participate were interviewed by telephone. Results: The response rate was 64% (n=77). Almost 38% of respondents reported that they used measures to identify patients with low literacy, most often during patient counselling. The most common strategy used to optimise the health care of patients with low literacy was spending extra time explaining the information. Written information in the form of Self Care cards and information leaflets was the resource most commonly available. However, 4% of respondents had no resources available in their pharmacy.

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  • The influence of the regional distribution of reduced lung elastic recoil on FEV1

    Hedges, KL; Hoffman, EA; Tawhai, MH (2010)

    Conference poster
    The University of Auckland Library

    Rationale: A link has been observed in several studies between the regional distribution of emphysema and the resulting magnitude of reduction in FEV1. The aim of this study is to examine how the loss of elastic recoil associated with emphysema that develops in localized regions of the lung results in the observed trends in FEV1 reduction.

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  • Preclinical characterization of PWT33597, a dual inhibitor of PI3-kinase alpha and mTOR.

    Matthews, DJ; O???Farrell, M; James, J; Giddens, Anna; Rewcastle, Gordon; Denny, William (2011-04-05)

    Conference poster
    The University of Auckland Library

    4485: Phosphoinositide-3-kinase (PI3K) is an important mediator of tumor cell growth, survival and proliferation. In particular, PI3K alpha is important for signaling downstream of receptor tyrosine kinases and is also frequently amplified or mutationally activated in tumors, suggesting that selective inhibitors of this isoform may have therapeutic utility in the treatment of cancer. Downstream of PI3K, the mTOR kinase also plays a critical role in cellular growth and metabolism, and inhibitors of mTOR have demonstrated clinical benefit in several tumor types. We report here the discovery and characterization of PWT33597, a dual inhibitor of PI3K alpha and mTOR. PWT33597 inhibits PI3K alpha and mTOR in biochemical assays with IC50 values of 19 and 14 nM respectively, and is approximately 10-fold selective with respect to PI3K gamma and PI3K delta. Profiling of PWT33597 against 442 protein kinases (Ambit Kinomescan) revealed little or no cross-reactivity with either serine/threonine or tyrosine kinases, and there was little cross-reactivity with an additional panel of 64 pharmacologically relevant targets. In NCI-H460 and HCT116 tumor cells with mutationally activated PI3K alpha, PWT33597 inhibits phosphorylation of PI3K and mTOR pathway proteins with cellular IC50 values similar to its biochemical IC50 values. PWT33597 has good pharmacokinetic properties in multiple preclinical species, is not extensively metabolized in vivo and shows little potential for interaction with cytochrome P450 enzymes. Following a single oral dose in vivo, PWT33597 shows durable inhibition of PI3K and mTOR pathway signaling in xenograft tumors. High compound distribution into tumors and potent anti-tumor activity has been observed in multiple tumor xenograft models with activated PI3K/mTOR pathways. Also, administration of PWT33597 in mice is associated with transient increases in plasma insulin, consistent with an effect on PI3K/AKT signaling. A robust PK/PD relationship has been defined, which will guide interpretation of the planned phase I clinical study. IND-enabling studies with PWT33597 are currently in progress.

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  • Design of a water soluble amethyrin macrocycle

    Albrett, Amelia; Brothers, Penelope; Aguilar, A; Lee, JT; Sessler, JL (2006-07)

    Conference poster
    The University of Auckland Library

    This JPP issue contains all abstracts accepted for presentation at the Fourth International Conference of Porphyrins and Phthalocyanines (ICPP-4) which was held in Rome, Italy, July 2-7, 2006 and coorganized by Drs Roberto Paolesse and Pietro Tagliatesta from the University of Rome ???Tor Vergata???. The printed book of abstracts was given to all ICPP-4 attendees, being a necessary tool to follow the rich scientific program of the meeting, and should also prove useful to those readers of JPP who were not able to attend the biennial scientific meeting of the Society of Porphyrins and Phthalocyanines (SPP).

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