2,407 results for Otago University Research Archive, Masters

  • Maximising the Potential of Existing Urban Infrastructure: Can Infrastructure Reuse Provide Successful Public Spaces?

    Kean, Gemma (2015)

    Masters thesis
    University of Otago

    There is great potential for innovative and creative public spaces beyond the traditional park or plaza to exist, yet this is still what most local authorities provide for in their public space policies. As cities intensify there is a need to provide additional public space in what may not have been considered to conventionally be a part of the public realm. Infrastructure is one example which can be used to provide additional public space through the adaptive reuse of a site, instead of abandonment or demolition when infrastructure is no longer required due to technological advancement. This research investigates whether the adaptive reuse of infrastructure can help create successful public spaces, and whether reuse can contribute towards improving the connectivity of an area. This is done using two case studies: Paddington Reservoir Gardens and the Ultimo Pedestrian Network in Sydney, New South Wales, Australia. The project explores whether there is too much focus on reusing the space with minimal adaptation, and the extent to which the planning processes enable or inhibit development, and allow for or discourage stakeholder involvement. The findings indicate that the adaptive reuse of infrastructure can provide interesting public spaces, however, success is dependent on the surrounding context. The two case studies employed in the research are vastly different. Despite this, the results show that infrastructural public spaces need to be active, provide for a range of users, and incorporate themes such as stickability and fine grain design to contribute positive outcomes to an urban environment.Often with infrastructure there is a risk of focusing too much on the preservation of heritage sites or making do with what already exists, instead of taking a greenfield approach to development. This can lead to spaces which are not integrated with the surrounds and which are not frequented or used as well as they could be. Further research needs to be undertaken to better understand the extent to which these particular public spaces-adaptively reused infrastructure differ to other spaces in the public realm.

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  • The Experience of Depression in the Tokelauan Culture in Two North Island Communities

    Loan, Iain Stuart (2015)

    Masters thesis
    University of Otago

    Background and Aims: The Tokelauan language lacks a word that corresponds to the western term ‘depression’. Furthermore, there is no research on the experience of depression in Tokelauans, and yet doctors continue to apply a western biomedical model of depression to Tokelauan patients and those from other Pacific cultures. This research aims to describe the experience of depression in Tokelauans and provide insight into its management. Better awareness of the symptoms and signs of depression as experienced by Pacific Islanders will enhance diagnosis and treatment of the illness by general practitioners. Method: Following extensive consultation with the Tokelauan community in Taupo, and using purposive stratified sampling, ten respondents contributed to this study. Semi-structured in depth interviews were performed and recorded verbatim. The transcripts of the interviews were thematically analysed using an immersion crystallisation technique, with further analysis to detect sub themes. Results: There is no specific word for depression in the Tokelauan language but an illness involving extraordinary sadness does exist. Ordinary sadness is regarded as just ‘part of life’ but extraordinary sadness can be classified as “unwellness” or “a burden". Tokelauans use several indicators to recognise someone with extraordinary sadness. The main indicator is isolation and withdrawal from family and community activities as well as absence from work and church. Tokelauan men are more likely to hold their feelings in and may indicate their unwellness with increased alcohol use or violent tendencies. For Tokelauans, privacy and pride are important cultural characteristics and these may be barriers to recognising sadness. The shame and loss of status associated with displaying sadness may also cause a person to hide his or her feelings. Often the smiling Tokelauan face becomes the mask that hides sadness. The main causes of extraordinary sadness are the changes caused by western influences on the Tokelauan culture and the stress of poverty and unemployment. The family, community and church are all important avenues for caring and for counselling the Tokelauan with extraordinary sadness. Discussion: This research documents some of the features of depression experienced by Tokelauans that are different from those that doctors may be trained to detect and manage using a western biomedical model. This research demonstrates the complexity of relationships between the patient, their illness and their culture that impacts on how the illness manifests. Similarly, this research indicates that therapy must have a holistic approach that includes the family, the community and that accounts for the patient’s spiritual beliefs. Te Vaka Atafaga is a metaphor for Tokelauan wellbeing involving a canoe. Its structure is representative of different components of health, and it provides a holistic model for the general practitioner involved in assessing and treating Tokelau Islanders with a possible depressive illness. The model does not exclude the use of western medical approaches, but it emphasises the need for social disharmony to be corrected to allow healing. Conclusion: The presentation and management of depression in Tokelauans may differ from that of other patients in a general practice setting. The Te Vaka Atafaga model provides the general practitioner with a tool to assess the different components that comprise health in the Tokelauan. A holistic approach involving the family, spirituality and correction of social factors along with palagi medicine is then necessary for treatment.

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  • A Qualitative Exploration of the Barriers and Enablers to New Zealand City Councils Developing and Implementing Food and Nutrition Policy

    Gower, Jacinda Ruth (2015)

    Masters thesis
    University of Otago

    Background: Diet- related non communicable diseases and obesity are the biggest cause of ill health and mortality in New Zealand. Current government public health approaches do not appear to be effective in preventing non- communicable diseases and obesity as rates continue to increase. To combat the obesity epidemic research suggests government regulative policy which positively shapes food environments is needed. New Zealand’s current government’s ideologies’ reject public health nutrition regulative policy so are unlikely to be an effective agency to reduce obesity and non- communicable disease rates. Local Authorities have been proposed as an alternative government organisation that has the ability to positively influence local food environments through developing and implementing food and nutrition policy. However, no New Zealand Local Authorities have food and nutrition policy and currently, there is no research regarding Local Authority food and nutrition policy in a New Zealand context. Objective: This research project aims to explore factors which enable, hinder and influence New Zealand City Councils’ ability to develop and implement food and nutrition policy. Methods: This public health nutrition study is set in a policy context so qualitative research was used to explore the social and organisational factors influencing City Councils’ development of food and nutrition policy that supports health food environments. Semi- structured in depth telephone and face-to-face interviews were carried out with 21 participants with representation from each of the 12 City Councils and Auckland Council. These interviews consisted of nine core questions which were informed by a review of the literature. All interviews were recorded and selectively transcribed. A general inductive approach was used to thematically analyse the data to categorise it into six major themes underpinned by minor themes. A single case study design was used to portray emerging themes and to understand the context of New Zealand City Councils’ capacity to develop food and nutrition policy. Results: The results of this study identified an array of factors which influenced City Councils’ decision making to develop and implement food and nutrition policy to improve local food environments. Six overarching categories emerged as being prominent to explaining City Councils’ capacity to influence food environments. These categories are council resources, community influence, political factors, long term plans, national-level governments and research, case studies and nutrition guidelines. All of these influencing factors had the potential to act as a barrier or an enabler dependant on the local political environment. The main finding is City Councils’ have the capacity to develop food and nutrition policy when there is a widespread awareness and prioritisation of food environment issues in the agenda of three key groups; the community, elected members of council and council staff. Conclusion: New Zealand City Councils capacity to develop and implement food and nutrition policy is determined by a host of external, internal, national and local influence factors. A multi pronged approach of strong local political support, partnerships, credible champions and local or case study research are needed for food environment issues to be addressed by City Councils through the LTP and subsequent food and nutrition policy. To achieve this New Zealand’s public health community need to be active advocates at a City Council level and be involved with activating communities and raising awareness around food and nutrition issues.

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  • Nutritional assessment of older New Zealand adults living in rest homes in the lower South Island

    Greenwood, Daniel (2015)

    Masters thesis
    University of Otago

    Background: The increasing numbers of New Zealanders aged over 65 years will place a large burden on healthcare resources and rest home facilities around the country. Malnutrition is a major contributor to adverse health outcomes in the elderly, leading to higher mortality, morbidity and lower quality of life. There is very little information on the prevalence of malnutrition among New Zealand rest home residents, and there is not any information on the adequacy of nutrient intakes in this population. However, international data show very high rates of malnutrition and poor nutrient intakes amongst elderly residents in long-term low-level care. Objective: The specific aims of the study are in residents of two rest homes in the Lower South Island: i) To describe the prevalence of inadequate nutrient intakes; ii) To describe the prevalence of malnutrition risk; and, iii) To describe the prevalence of anaemia. Design: This cross-sectional study included 35 participants (14 men and 21 women), aged 69-102 years who lived in 2 rest homes in the lower South Island. Information on demographics, medical history, medications and supplement use were collected from medical notes. Malnutrition screening was done using two different screening tools- Mini Nutritional Assessment-Short Form and Malnutrition Universal Screening Tool. Cognitive function and mood were examined using the Clock Drawing Test and the Geriatric Depression Scale. Anthropometry measurements collected were; height, ulna length, weight, using standard protocols and body mass index (kg/m2) was calculated using average ulna length measurements. Dietary intake data were collected with 3-day food records, over 2 non-consecutive week days and one weekend day. Food intakes were matched to nutrient lines in the New Zealand Food Composition Tables to determine nutrient intakes. Nutrient intakes were then compared with current recommendations to estimate the prevalence of inadequate intakes. Blood and urine samples were taken for later analysis of biochemical nutritional status. Ethical approval was granted by the University of Otago Human Ethics Committee (Health) (H13/118). Results: The duration of stay in the rest homes ranged from 4 to 161 months (mean = 47months. Fifty percent of participants had a BMI over 25kg/m2, and 11% were underweight (BMI <20 kg/m2). Overall energy intakes were low, with 43% of men and 76% of women having suboptimal energy intakes (P=0.046 for differences between men and women). Sixty-three percent had inadequate protein intakes. Mean saturated fat intake was high, (16% of total energy intake), and average fibre intakes were low (19 g/day). All participants had suboptimal selenium and vitamin D intakes, although 83% of participants were on a monthly vitamin D supplement. Over 90% had inadequate intakes of calcium, magnesium, vitamin B6 and vitamin E, and over 20% had inadequate intakes of thiamin and vitamin B12. When assessed with the MNA-SF tool, 53% were classified as being at risk of malnutrition. When using the MUST screening tool, 39%, were classified as being at risk of malnutrition. Anaemia rates were high in both men and women (57%). Conclusion: We have shown that malnutrition and inadequate micronutrient intakes are prevalent in rest home residents in the lower South Island. More research and strategies are needed to ensure that rest home residents are gaining the appropriate level of nutrition required to stay healthy and functional for as long as possible. 

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  • He kupu tuku iho mo tenei reanga : Te ahua o te tuku korero

    Higgins, Rawinia Ruth (1999)

    Masters thesis
    University of Otago

    170 leaves ; 30 cm. Includes bibliographical references. University of Otago department: Māori, Pacific and Indigenous Studies. "March 1999."

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  • The development of Otago's main road network

    Baker, Neill Reginald (1969)

    Masters thesis
    University of Otago

    x, 112 leaves :ill. (some col.), maps ; 30 cm. Includes bibliographical references. University of Otago department: Geography.

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  • Telling our professional stories

    Alterio, Maxine (1998)

    Masters thesis
    University of Otago

    [6], 138 leaves :ill. ; 30 cm. Includes bibliographical references.

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  • Larval drift and development of amphidromous fishes, particularly the bluegill bully (Gobiomorphus hubbsi)

    Jarvis, Matthew Graham (2015)

    Masters thesis
    University of Otago

    Amphidromy is a distinct life history strategy found in many aquatic organisms, involving a return migration (‘drift’) to a pelagic feeding habitat (usually the sea), undertaken by newly hatched larvae. The freshwater fish faunas of many Indo-Pacific islands are dominated by amphidromous species, yet they remain understudied, especially their larval stages. Amphidromous larvae hatch out exceptionally small and undeveloped, and so face a range of specific challenges during migration such as irreversible starvation and failed development if migration is delayed, as well as management difficulties due to their small size. Basic ecological knowledge such as timing and extent of migration remains unknown, but is crucial to the management of amphidromous species. It was therefore the aim of this thesis to further our knowledge on the larval ecology and migration of a number of New Zealand’s amphidromous fish species. This thesis examines patterns of larval drift and development, focussing on the bluegill bully (Gobiomorphus hubbsi), an endemic eleotrid. A distinct diel and spatial drift pattern was documented, with the vast majority of fish larvae migrating to sea within a few hours of sunset. It is suggested that targeting conservation measures within this window of drift represents a potentially beneficial management strategy for amphidromous species. Development and starvation of larvae was also examined, both through field studies and lab experiments. No distinct pattern of starvation was observed in larvae during their seaward migration, however larvae retained in freshwater failed to develop to as complete a state as those transferred to seawater, implying delayed migration may adversely affect amphidromous fishes developmentally, ultimately reducing their success upon reaching the sea. These results indicate both threats to amphidromous fishes during their larval migration, and a potential approach which may prove beneficial in conserving these fascinating and vulnerable species.

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  • The X-ray Crystal Structure of Alanine Racemase from Acinetobacter baumannii

    Davis, Emily (2015)

    Masters thesis
    University of Otago

    Acinetobacter baumannii is an opportunistic Gram-negative bacterium, which is a common cause of hospital acquired infections. Numerous antibiotic resistant strains exist, emphasising the need for developing new antimicrobials. Alanine racemase is a pyridoxal 5’-phosphate dependent enzyme, responsible for racemisation between enantiomers of alanine. As D-alanine is an essential component of the bacterial cell wall, its inhibition is lethal to prokaryotes, making it an excellent antibiotic drug target. In this study, A. baumannii alanine racemase (AlrAba) was cloned and expressed in Escherichia coli. A purification protocol was then developed involving ammonium sulphate fractionation and chromatography steps (Hydrophobic interaction, anion exchange and size exclusion). This purification protocol was able to produce 11.5 mg/L of AlrAba with a purity of greater than 95 %. The kinetic parameters of AlrAba were determined using spectrometric coupled enzyme activity assays. The Vmax and Km for the L-alanine to D-alanine reaction were found to be 220.5 U/mg and 1.56 mM, respectively. The Vmax and Km for the D-alanine to L-alanine reaction were found to be 11.3 U/mg and 0.56 mM, respectively. AlrAba was successfully crystallised and the structure was determined using X-ray crystallography. The structure was initially solved to 1.9 Å resolution via molecular replacement using the monomer of Pseudomonas aeruginosa alanine racemase as the search model. The final structure had an Rfactor of 19.7 % and an Rfree of 23.4 %. The resolution was then extended to 1.65 Å with an Rfactor of 20.6 % and Rfree of 23.6 %. The tertiary structure AlrAba was established to be a homodimer, in which the two monomers interact in a head to tail manner, resulting in two active sites per enzyme. Each active site is comprised of residues from the N-terminal domain of one monomer and the C-terminal domain of the second monomer. The N-terminal domain corresponds to residues 1 – 230, and consists of an eight-stranded α/β barrel. The C-terminal domain corresponds to residues 231 – 356, and mainly contains β-strands. Comparison of AlrAba with alanine racemases from closely related bacteria demonstrated a conserved overall fold, substrate entryway and active site. The structure of AlrAba will provide a template for future structure-based drug design studies.

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  • Living in Two Cities: Lessons for the church today from Augustine's City of God

    Broome, Deborah Louise (2015)

    Masters thesis
    University of Otago

    Reading Augustine’s City of God through the lens of public theology, as well as in conversation with some of his leading commentators, provides an opportunity to discover how Augustine’s account of the two Cities (the civitas Dei and the civitas terrena) might inform the role of Christians in contemporary New Zealand, those who are living both as citizens in temporal society (the secular realm) and members of an alternative, Christian, society. There are parallels between Augustine’s society and our own which make a reading of City of God instructive for the Church today. The occasion of Augustine’s writing of City of God is briefly discussed, as is the theme of the two cities in the Bible and elsewhere in ancient literature. Attention is given to the nature of public theology and significant issues which public theologians must address, including the location of the debate (a secular public square), the language used, and the right to speak. A key notion is ‘seeking the welfare of the city’. Augustine is considered as public theologian and as apologist. The structure of City of God is analysed, key themes considered, and a précis offered which focuses on Augustine’s treatment of the two cities throughout the work. The nature of the City of God and the Earthly City are examined, in discussion with major commentators: the cities are societies defined by their members and by what their members love. The Church is not the City of God, but is rather a sign and an anticipation of it. Likewise, the Earthly City is not Rome, nor the State. The two cities are interwoven and intermixed, perplexae and permixtae with one another, and interact in this present age, in the saeculum. Central to Augustine’s thinking is that members of the City of God on pilgrimage in the world should not withdraw from that world but be involved in its society and institutions. Ways in which Christian communities might engage with the surrounding culture are examined, including the idea of work as loving service; and a number of lessons for the Church today are drawn. Dealing with ‘the other’ and encountering diversity are important issues. The relationship between the Church and the State is considered, as is the nature of the Church as public space in its own right. A deeper relationship between Christian faith and public engagement is encouraged. Central to the application of City of God to our current setting is the idea of the citizens of the civitas Dei on pilgrimage, and what it means to be part of a pilgrim city. Viewing City of God through an eschatological lens is crucial. A major conclusion is that ‘living in two cities’ is not merely a description of what it feels like for Christians today: it is an indication of how our life is actually meant to be.

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  • The Impact of Ocean Acidification on Parasite Transmission

    Harland, Hannah (2015)

    Masters thesis
    University of Otago

    This study examines how ocean acidification affects parasite transmission. Ocean acidification is a global process which has already started to have negative impacts on the marine environment, and these are predicted to escalate with future acidification. These include impacts on reproduction, development, calcification, osmotic regulation, and survival. Parasites are key components of all marine ecosystems, influencing the survival of their hosts, and also indirectly affecting other species within marine communities. Many parasite species possess vulnerable life history stages, which are sensitive to abiotic changes. Intertidal parasites have therefore been proposed as good bio-indicators for the impacts of ocean acidification. This research used Maritrema novaezealandense and its first and second intermediate hosts as a model system to look at the impact of ocean acidification on parasite transmission. It was hypothesised that acidified conditions would affect transmission from the Zeacumantus subcarinatus snail host to the Paracalliope novizealandiae amphipod host. Parasite transmission was tested under three pH levels (pH 7.4, pH 7.6 and pH 8.1) and infection success within amphipod hosts was determined. Parasite infections in amphipods were significantly higher at the pH 7.4 level. Infection by this parasite may therefore increase with future ocean acidification. Amphipods were more vulnerable to parasitism under seawater acidification and may be the weak link in this model system. To see whether parasite genotypes vary in their sensitivity to acidified conditions, the transmission success of eight different parasite genotypes was examined. Genotype was not found to significantly impact infection success, with pH level being the main determinant of infection success, regardless of genotype. The virulence of parasite genotypes did vary, however, with some genotypes inducing greater amphipod mortality following infection. Parasites which are less virulent may therefore have an increased chance of reaching the definitive host and this could be particularly important when this sensitive amphipod species is faced with both the stress of parasitism and ocean acidification.

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  • Reconsidering The Nonhuman Animal: A Multidisciplinary Approach

    Muirhead, Eliza Kate (2015)

    Masters thesis
    University of Otago

    Nonhuman animals exist to humans in a myriad of different ways. As companions or entertainers, as tools for scientific endeavour, within the natural environment and lastly, for the majority of people they exist as a source of consumable food or materials. To study the human-animal relationship, as it is becoming increasingly popular to do, is to confront the complexity of these relations. The popularity of such an endeavour is exemplified by the rise of a relatively new discourse of academic enquiry called human animal studies (HAS). HAS places the nonhuman animal in the spotlight of a multidisciplinary discussion which explores the question of what the human relationship with nonhuman animals ought to be. However, before this question can be posed, we must first understand the rich and interconnecting history of epistemology that has formed our contemporary ‘way of knowing’ the nonhuman animal. As a result of examining how certain disciplines have sculpted our contemporary understanding of the nonhuman animal we can also demonstrate the necessity of a multidisciplinary approach. It suggests that without a dialogue between particular fields, such as philosophy and science, we are limited in our ability to construct a set of ethics that may articulate what our proper relationship with nonhuman animals ought to be. This thesis provides a brief overview of the epistemology that has formed our current understanding of this question and situates the discussion within the field of science communication. In much the same way that the field of deep ecology first suggested in the 1980’s, the field of science communication suggests that in order to bring in to question the contemporary ‘way of knowing’ the nonhuman animal and therefore our current use and treatment of them, we must create a dialogue between the theoretical, social, political and historical (Naess 1984). This dissertation will review areas where a disconnect between the fields of science and philosophy have resulted in producing ‘untruths’ in the way that we ‘know’, ‘value’, ‘think’ and therefore ‘act’ for and ‘represent’ the nonhuman animal. It will show that there is a disconnect between what we know about the nonhuman animal through science, on their intelligence, ability to experience the world, and the way that ethics have developed to guide in how we ought to treat the nonhuman animal. The artefact component of this dissertation, Human|Animal a 25min documentary, is a reply to this call. It acts as a piece of science communication and aims to create a personal response in the audience in order to elicit a re-evaluation of the current way in which the nonhuman animal is utilised in western society. By engaging in a multidisciplinary dialogue the film asks the audience to consider, and potentially form an opinion, on what our current treatment of the nonhuman animal ought to be.

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  • Targets of the QseM Antiactivator in Mesorhizobium loti

    Major, Anthony Scott (2015)

    Masters thesis
    University of Otago

    Quorum Sensing (QS) is a system used by bacteria to coordinate gene expression in response to population density using secreted diffusible signalling molecules, known as autoinducers. Many QS systems are similar to the model LuxR/I system originally discovered in Vibrio fischeri, where constitutive expression of the autoinducer synthase luxI produces acyl homoserine lactone molecules (AHLs) known as autoinducers at low levels. Once the population density reaches a threshold level, the regulator LuxR recognises and responds to the AHLs, activating downstream gene expression. These systems may also involve an antiactivator, that acts on the LuxR protein to prevent premature activation of the system by low AHL levels. Mesorhizobium loti strain R7A contains a mobile 502-kb symbiosis island known as ICEMlSymR7A which can transfer to nonsymbiotic mesorhizobia in both the laboratory and the environment. The excision and transfer of ICEMlSymR7A is directly controlled through QS via the actions of the the regulator TraR that acts in conjunction with AHL made by the autoinducer synthase TraI1. TraR activity in turn is controlled by the antiactivator QseM, through direct interaction with the TraR+AHL signalling molecule complex to block promoter activation. In this work, RT-qPCR was used to demonstrate that QseM had an effect on downstream TraR-regulated gene expression. Strong expression of the ICEMlSymR7A excisionase gene rdfS or the TrbC protease gene traF is known to have an inhibitory effect on cell growth. These genes are regulated by QS through the intermediacy of the msi172-msi171 gene product which is a single protein, FseA, that is produced by frame-shifting. A conjugation-based growth-inhibition assay involving introduction of a potentially lethal plasmid overexpressing target proteins into cells either overexpressing or not expressing QseM was developed to detect targets of QseM. The assay confirmed that TraR was a target of QseM and further suggested that FseA was a further target. RdfS and TraF were eliminated as targets. Bacterial two-hybrid analyses confirmed FseA as a target and narrowed the interacting portion down to the Msi172 portion of the frame-shifted protein. Furthermore β-galactosidase assays showed that FseA was unable to activate the rdfS promoter in the presence of QseM. Overall, this work confirmed the role of QseM as an antiactivator within the ICEMlSymR7A transfer system regulatory network and revealed it has more than one target. A 6-His tag was attached to QseM and a high concentration of protein was purified. Attempts at determining QseM interacants through Mass Spectrometry from a R7AΔqseM lysate proved difficult despite distinct bands being seen. QseM was subjected to circular dichroism that inferred that QseM is composed solely of α-helices, as is TraM, an antiactivator that targets TraR from the Agrobacterium tumefaciens QS system.

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  • Supervision and the culture of general practice

    Wilson, Hamish John (1999)

    Masters thesis
    University of Otago

    Supervision is a well-known and well-theorised activity in some professions where experienced practitioners contract to facilitate, guide or educate the novice. Supervision is uncommon in medicine, which has traditionally employed more didactic teaching processes. In the general practice community in New Zealand, practitioners use a variety of methods of professional maintenance, with educative mentoring or supervision being a recent innovation. In this form of supervision, general practitioners (GPs) discuss their work with an experienced supervisor, with one focus being to learn counselling or psychotherapy skills. This study examined the experiences of GPs who use supervision, with particular reference to how supervision impacts on their practice of medicine. The context for this inquiry included the background philosophical assumptions of the biomedical paradigm, current problems in clinical practice and the culture of general practice in New Zealand. The research used a qualitative methodology, with seven GP respondents being interviewed at length about their use of supervision. A focus group with four of the participants followed initial analysis of the individual interviews. Interviews and group discussion were analysed within a social constructivist paradigm. The respondents' stories of learning about supervision led to the construction of a collective story. This could be outlined under the four broad themes of dissonance and exploration, self-awareness and professional development, the supervised practice, and defining supervision in general practice. However, before these GPs could make effective use of supervision, they needed to work through a number of personal and cultural barriers. The findings of the research suggest that supervision is a powerful method of learning, being an embodied experience through the supervisor-doctor relationship. Some of these GPs used their supervisor to learn how to do psychotherapy in general practice. The supervisor also acted as sounding board for all the respondents to discuss other work issues, such as practice management and peer relationships. One outcome of regular supervision was validation about their work, contributing to a heightened sense of self in the work environment. Supervision facilitated a model of reflective learning that is relatively uncommon in medicine. This was achieved through rigorous attention to self-awareness, resulting in facilitated career development. In a supervised practice, the GP incorporates an increasing acuity for patients' psychological problems. There is an emphasis on the doctor-patient relationship, with awareness of the roles and boundaries around the GP’s work. Supervision was seen to be different to work in peer-groups or in personal psychotherapy, but there were similarities. The role of the supervisor was defined to include sub-roles of teacher, facilitator, analyst and evaluator. In this study there was invariably no form of summative evaluation. The results led to a definition of supervision in general practice. Studying these successful supervisor-doctor relationships gave unique insights into the barriers that prevent further utilisation of supervision or other forms of mentoring in general practice. These barriers include broad issues of the traditional epistemological assumptions of modern medicine. Having supervision appeared to have a major impact on the style of medical practice that is exhibited by these GPs. There are implications of these findings for both undergraduate and postgraduate medical education. This research was grounded in a social constructivist paradigm that linked theory, research and clinical practice. From the evidence presented here, these practitioners have incorporated biomedicine into a wider medical model that offers a resolution to the current paradigmatic crisis of modern medicine.

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  • Environmental tolerances of resting stages of plumatellid bryozoans at Southern Reservoir, Dunedin, New Zealand

    Brunton, Michelle A (2005)

    Masters thesis
    University of Otago

    Freshwater bryozoans grow at Southern Reservoir, a water treatment station in Dunedin, New Zealand fouling surfaces in the microstrainer hall and requiring expensive and time consuming maintenance. Preventing or minimising germination is a potentially useful way to control bryozoan infestation. Germination trials were conducted to assess the effect of temperature, dry storage, and various chemicals on floatoblast germination. Plumatella vaihiriae is newly described from Southern Reservoir. A second, as yet unidentified, Plumatellid species was discovered through the presence of floatoblasts (Plumatella n. sp.). Storing dry floatoblasts of Plumatella n. sp. decreased germination. Storing dry floatoblasts of P. vaihiriae for 63 days or more prevented germination entirely. Sodium hydroxide, acid clean in place (CIP) and sulphuric acid were most successful of those chemical treatments tested in preventing germination of P. vaihiriae floatoblasts. Exposure to alkaline CIP and a combination of CIP solutions decreased germination of P. vaihiriae floatoblasts. Pre-heating acid CIP to 35°C prevented germination of P. vaihiriae floatoblasts. Exposure to alkaline CIP solutions at temperatures of 35°C and 50°C increased germination of P. vaihiriae and Plumatella n. sp. floatoblasts. The zooid wall provided protection for floatoblasts of P. vaihiriae from exposure to acid CIP. On initial release from the zooid P. vaihiriae floatoblasts produced in winter and early summer are obligate dormants entering a diapausal state for at least three weeks. As summer approaches P. vaihiriae floatoblasts become thermos-quiescent and germinate once temperatures increase. Dunedin City Council (DCC) water department staff must adhere to strict cleaning policies when moving both people and equipment between Southern Water Treatment Station and other water treatment stations and reservoirs. P. vaihiriae and Plumatella n. sp. floatoblasts could better sustain the effects of an acid environment during transport within a vector such as a water bird more so if eaten whilst within the zooid and remaining within the zooid walls during transport.

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  • A Study of Labour Negotiators: Orientation and Behaviour

    Ferguson, Kelly (2015)

    Masters thesis
    University of Otago

    This exploratory study examined individual negotiator social value orientation (preferences for the distribution of negotiated outcomes) and individual negotiator behaviour (strategies and tactics) in a labour relations context. Interviews were conducted with professional labour-management negotiators and collective bargaining negotiations were observed. The findings reveal that the majority of negotiators are competitively oriented and that a number of negotiators have a mixed orientation (both competitive and collaborative). Furthermore, the study reveals that distributive strategies and tactics dominate in real-world negotiations. Most of the negotiators were found to adopt a distributive strategy exclusively. However, the study also revealed that a number of negotiators utilise both distributive and integrative behaviours (albeit within a predominantly distributive strategy). Furthermore, the study examined the rigour with which behaviours are implemented. Since distributive strategies and tactics were found to be dominant it was not possible to analyse the rigour of integrative behaviours. Notwithstanding, the strength of distributive strategies and tactics were analysed. The findings show that negotiators implement distributive tactics from along a continuum that ranges from “hard” to “soft”. In fact, the majority of negotiators were found to be operating at some mid-point along that continuum, adopting a “moderate” approach to distributive bargaining that was neither hard nor soft but fell somewhere in-between. Finally, this study considered whether orientation predicts negotiation behaviour. The findings show that competitively oriented negotiators adopt distributive strategies and tactics almost exclusively, whereas the negotiators with a mixed orientation were found to be far more likely to adopt some integrative behaviour (even though their overall approach is predominantly distributive). As mentioned, the findings reveal that distributive behaviours are implemented with different degrees of rigour. Competitively oriented negotiators were found to engage in hard, moderate and soft distributive bargaining. The majority of cases were categorised as moderate, but hard and soft approaches exist. In contrast, negotiators with a mixed orientation were found to implement a moderate distributive approach only. The implications for this research and avenues for future research are discussed.

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  • Therapeutic Approaches in the Attenuation of Seizure-Induced Cardiomyopathy

    Andreianova, Anastasia Alexeevna (2015)

    Masters thesis
    University of Otago

    Single subcutaneous administration of Kainic acid (KA) in the rat produces significant levels of seizure activity, including head tremors, salivation and tonic-clonic convulsions. Using electrophysiological quantitative techniques which measure electroencephalographic (EEG) as well as electrocardiographic (ECG) trace activity following KA administration, the effects of seizure activity on the function of the heart were assessed over a 48 hour period. In addition, histopathological analysis was carried out in order to determine whether the ongoing seizure activity produced significant changes in ventricular myocardium indicative of irreversible cardiomyopathy. In order to determine the potential mechanism of action of KA-induced cardiac damage, a further two animal groups were examined. The groups consisted of animals pretreated with either atenolol or clonidine. The two different drugs were used in order to isolate systems involved in cardiac damage, where atenolol acts specifically in the periphery, while clonidine is known to act in the central nervous system. Analysis of EEG and concomitant ECG traces, during and following seizure activity demonstrated significant changes in heart rate (HR) as well as associated HR parameters compared to baseline. Upon further histological observations it was apparent that at 48 hours following KA administration, ischaemia was present as well as evidence of inflammatory cell infiltration, tissue tearing and oedema compared to saline treated animals. Further assessment of pretreated animal groups lead to the conclusion that atenolol was not protective against KA-induced cardiac damage in the rat while clonidine was. These findings propose that the mechanism by which KA-induced seizure activity results in cardiomyopathy is through modulation of brain centres associated with cardiac control, as opposed to KA binding to peripheral cardiac receptors as previously suggested.

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  • W. E. Gudgeon : his contribution to the annexation of the Cook Islands.

    Currie, Ernest Rowland (1963)

    Masters thesis
    University of Otago

    v, 90 leaves ; 30 cm. Bibliography: leaf iv-v.

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  • How to be a Prehistoric Weatherman: Using n-alkanes as a Proxy for Holocene Climate and Hydrology, Southwest South Island, New Zealand

    Burrington, Peter (2015)

    Masters thesis
    University of Otago

    The latitudinal position and strength of the Southern Hemisphere westerly winds (SHWW) plays a critical role in global CO2 air-sea flux and the distribution of rainfall in the southern mid-latitudes. Strengthening and southward shifting westerlies are thought to be reducing the efficiency of the Southern Ocean carbon sink, which has direct implications for modern atmospheric CO2 concentrations. Southern New Zealand intersects the northern margin of the SHWW belt, where a direct relationship exists between annual precipitation and zonal wind speeds. Reconstructing past hydrological variations from the southwest of the South Island (SWSI) can provide a regional record of climatic response to changing SHWW. A 5.4 m sediment core spanning the last 10.8 ka was recovered from South Mavora Lake, Southland. Magnetic susceptibility, bulk organic C and N isotopes and concentrations, and lipid biomarker (C21-C33 n-alkanes) concentrations, distributions, and hydrogen isotope values (δD) serve as proxies for change in lacustrine productivity, relative proportions of terrestrial and aquatic input, and hydrology. Modern SWSI meteoric water isotope values (δ18O, δD) collected over a 12-month period, and meteorlogical station data, show orographic rainout and air temperature are the primary drivers of hydrological isotope composition in SWSI. Downcore interpretation of data suggests a period of increased precipitation, rapid warming, and greater terrestrial input from 10.8-9.0 ka, likely corresponding to weaker westerly influence over SHWW. From 9.0-7.0 ka, decreasing δDn-alkanes shows gradual cooling, δ13C and ACL suggest increased aquatic productivity, and stratigraphy shows an increase in storm strength. From 7.0-5.1 ka δDn-alkanes and δ13C are characteristic of a relatively stable temperate climate, Paq and C/N ratios suggest a relatively humid environment, and stratigraphy showed an increase of storm events. From 5.1-3.6 ka δDn-alkanes showed a large cold excursion followed by gradual warming, Paq and stratigraphy reflected a significant increase in storm event frequency and strength, and an increase in ACL reflected the expansion of cool-moist Nothofagus menziesii into the region. From 3.6 ka to present δDn −alkanes showed a cooling trend to present day, likely related to strengthening of the SHWW, and low amplitude and frequency variation in Paq and decreased storm events signaled a gradual decrease in precipitation to modern day conditions.

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  • Scanning Electron Microscopy of the Early Life Stages of the New Zealand Yellowfoot Paua, Haliotis australis and Factors Affecting Settlement.

    Maxwell, Paul Douglas Ian (2015)

    Masters thesis
    University of Otago

    The purpose of this research was to spawn and settle larvae of the Yellowfoot Paua (Yellowfoot abalone) Haliotis australis (Gmelin, 1791) to investigate the early life stages of H. australis and the factors influencing settlement of the larvae of this abalone species in a commercial aquaculture context. Scanning electron microscopy (SEM) was used to investigate the morphological aspects of the development of the life stages of Haliotis australis from gametes, veliger larvae, post larvae and juveniles to 60 days post settlement (70 days post fertilisation). The photo micrograph results presented in this thesis represent the first comprehensive SEM micrograph record of the early life stages of H. australis. Settlement experiments tested success of larval settlement on four different diatom biofilm settlement substrates; 8-Day [old] Ungrazed Biofilm, 8-Day Grazed Biofilm (pre-grazed by conspecific adults to produce mucus trails), 1 Day Biofilm and No Biofilm. Half of the experimental replicates were settled with H. australis larvae treated with gamma(γ)-aminobutyric acid (GABA) to test the effectiveness of GABA as a chemical treatment to enhance settlement of H. australis larvae. Post larval survival at 33 days post settlement was used to infer the settlement success occurring at the time of settlement (between Day-0 and Day-4 post settlement). Analysis of mean survival data at 33-days post settlement identified that established diatom biofilms pre-grazed with conspecific adults (8 Day Grazed Biofilms) produced higher settlement than one day old diatom biofilm (1 Day Biofilm) and No Biofilm (control) treatments. Established diatom biofilms (‘8 Day Ungrazed Biofilm’) produced higher settlement than ‘No Biofilm’ treatments. The differences observed were statistically significant. The analysis of mean survival at 33-days post settlement identified that there was no significant difference detected between GABA and no-GABA treatments. However the observed results indicated that 8-Day Ungrazed Biofilms and 8-Day Grazed Biofilms treated with GABA, and untreated (no-GABA) 8 Day Grazed Biofilm settlement surfaces produced the best inferred settlement of H. australis larvae when compared to untreated 8 Day Ungrazed Biofilm and both GABA and no-GABA 1 Day Biofilm and No Biofilm treatments. These results suggest that pre-grazing of prepared diatom film settlement substrates with conspecific adult or juvenile abalone may be employed by hatcheries to ensure the highest rates of settlement in H. australis larvae. When the pre-grazing of established diatom biofilms is not a practical option in a larger scale aquaculture context, then the treatment of competent H. australis larvae with a GABA solution prior to settlement may be used to enhance the success of settlements onto established diatom biofilms.

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